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Thermo|invitrogen|Qubit 4.0熒光計現(xiàn)貨
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Thermo|invitrogen|Qubit 4.0熒光計現(xiàn)貨
The Qubit 4 Flurometer is the latest version of the popular Qubit fluorometer designed to accuray measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. The Qubit 4 Fluorometer was re-engineered to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accuray distinguish intact from degraded RNA in just two easy steps.
For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
Key features of the Qubit 4 Fluorometer include:
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Exports data to a USB drive or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
The Qubit 4 Flurometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.
Enables more accurate results than with UV absorbance at low concentrations
The Qubit 4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accuray measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.
Fast, simple, and accurate measurement of RNA quality
The Qubit RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.
Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a USB thumb drive and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification.
Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 ?L thin-walled PCR tubes (Cat. No. Q32856) are required.
System specifications include:
Instrument dimensions: 5.4"(w) x 10" (l) x 2.2" (h) (13.6 cm x 25 cm x 5.5 cm)
Weight: 743 g
Dynamic range: 5 orders of magnitude
Processing time: ≤seconds/sample
Light sources: blue LED (max ~470 nm), red LED (max ~635 nm)
Excitation filters: blue (430–495 nm), red (600–645 nm)
Emission filters: green (510–580 nm), red (665–720 nm)
Detectors: photodiodes, measurement capability from 300–1,000 nm
Warm-up time: <35 seconds
USB thumb drive: 4 GB
For Research Use Only. Not for use in diagnostic procedures.
Thermo|invitrogen|Qubit 4.0熒光計現(xiàn)貨
靈敏,準(zhǔn)確,經(jīng)濟(jì)實(shí)惠
Qubit® 3.0熒光定量儀為全新升級的臺式熒光計,配套高靈敏的Qubit®定量分析試劑盒,精確定量DNA,RNA和蛋白質(zhì)濃度。采用專門研制的熒光染料,只有與樣品中的靶分子特異結(jié)合時方可發(fā)射熒光信號,從而報告靶分子的濃度,因此這種特異性可以使您獲得比傳統(tǒng)紫外吸光法更加精確的結(jié)果。
Qubit® 3.0熒光定量儀主要特點(diǎn)包括:
靈活選擇操作語言,包括英語,簡體中文,法語,西班牙語,德語,意大利語及日語
Qubit® 3.0 熒光定量儀尤其適用于:
■ 您的樣品十分稀有且難以處理
■ 提取后的dsDNA,oligos,RNA(包括microRNA)或蛋白質(zhì)量很少
■ 該樣品將被用于成本昂貴的下游實(shí)驗(yàn)
■ 這些樣品將用于實(shí)時定量PCR(qPCR)或下一代測序法(NGS)等需要精密測定的實(shí)驗(yàn)
■ 接下來要進(jìn)行轉(zhuǎn)染或其他應(yīng)用,需要幾天或幾周才能獲得結(jié)果的實(shí)驗(yàn)
■ 您的樣品制備過程復(fù)雜,需要激光顯微切割(LCM)等特殊技術(shù)
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