ABI/實(shí)時(shí)熒光定量PCR儀/4376598/現(xiàn)貨

簡(jiǎn)要描述：ABI/實(shí)時(shí)熒光定量PCR儀/4376598/現(xiàn)貨The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users.

產(chǎn)品型號(hào)：StepOnePlus
廠商性質(zhì)：經(jīng)銷商
更新時(shí)間：2018-01-05
訪問量：1873

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ABI/實(shí)時(shí)熒光定量PCR儀/4376598/現(xiàn)貨

產(chǎn)品介紹：

ABI/實(shí)時(shí)熒光定量PCR儀/4376598/現(xiàn)貨

規(guī)格

Calibrated Dye:	FAM™, JOE™, NED, ROX™, SYBR® Green I, TAMRA™, VIC®
Capacity:	1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
Detection Method:	Primer-Probe Detection, SYBR
Dimensions:	24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
Dynamic Range:	Linear Dynamic Range greater than 9 log units (detection)
External Computer:	Notebook
For Use With (Equipment):	StepOnePlus™
Format:	0.1 ml tubes, 8-tube strips, 96-well plate
Green Features:	Sustainable packaging
High Throughput Compatibility:	Multiplexing
Optics:	4 emission filters, Blue LED excitation source, photodiode
Passive Reference Dye:	No ROX, ROX (Pre-mixed), ROX (Separate Tube)
Peak Block Ramp Rate:	4.6°C⁄sec
Product Line:	StepOnePlus™
Product Size:	1 system
Reaction Speed:	Fast, Standard
Reaction Volume Range:	10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
Run Time:	<2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
Sample Ramp Rate:	Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
Sensitivity:	1 copy
Temperature Accuracy:	0.25°C (35 to 95°C) of display temperature
Temperature Range (Metric):	4-100°C
Temperature Uniformity:	0.25°C (35 to 95°C) of setpoint/display temperature
Thermal Cycling System:	Peltier-Based System
Weight:	24 kg (53 lbs.)

描述

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications. We supply a Dell™ Laptop with the StepOnePlus™ Real-Time PCR System.

Features of the StepOnePlus™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage

New StepOne™ Software
The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediay and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading
The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

The StepOnePlus™ System supports many Real-Time PCR applications, including the following:

• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods, including the following:

• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
• High Resolution Melting (as a standalone application)

Versatile Instrument Configuration Options
The ultra-compact footprint of a StepOnePlus™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOnePlus™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOnePlus™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.

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